transcriptional heterogeneity in naive and primed human

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The metabolome regulates the epigenetic landscape during naive-to-primed human embryonic stem cell transition Published in Nature Cell Biology 2015 Integrated trancriptomic epigenomic and metabolomic data and discovered that NNMT a metabolic enzyme regulates H3K27me3 methylation levels in naive to primed transition Endogenous retroviruses drive transcriptional innovation in human cancer: Creator: Artem Babaian: Publisher: University of British Columbia: Date Issued: 2019: Description: Transposable element (TE) exaptation is the process of TE incorporation into functional and in some cases necessary genes or regulatory units over evolutionary time

Transcriptional Heterogeneity in Naive and Primed Human

Cell Reports Report Transcriptional Heterogeneity in Naive and Primed Human Pluripotent Stem Cells at Single-Cell Resolution Tobias Messmer 1 2 7 Ferdinand von Meyenn 3 4 7 8 Aurora Savino 3 Fatima Santos 3 Hisham Mohammed 3 9 Tin Long Lun 1 *John C Marioni 1 5 6 * and Wolf Reik3 5 10 1Cancer Research UK Cambridge Institute University of Cambridge Cambridge CB2 0RE UK

GM-CSF production is associated with human T H 1 but not T H 17 cell regulation Pathogenic properties of T H 17 cells have recently been associated with GM-CSF production in murine autoimmune diseases () To explore whether GM-CSF production by human T cells is also regulated by the T H 17 cell axis we isolated T H 1 T H 2 and T H 17 cell subsets from the peripheral blood of healthy donors

Naїve Human Pluripotency The Broad Shoulders of Science Stevenage UK BioScience Campus Scientific debate in the pursuit of knowledge by way of accumulated evidential data is fundamental just as socio-economic competition is needed to spur innovation product development growth in commercial business

First naive human cells may offer an enhanced starting point for differentiation into disease-relevant cell types overcoming the heterogeneity frequently observed in current human ESCs and iPSCs Second the isolation of naive human cells may provide a cell culture system to study epigenetic mechanisms of human pre-implantation development that cannot be investigated in primed cells

Tet1 Tet2 and Tet3 encode DNA demethylases that play critical roles during stem cell differentiation and reprogramming to pluripotency Although all three genes are transcribed in pluripotent cells little is known about the expression of the corresponding proteins Here we tagged all the endogenous Tet family alleles using CRISPR/Cas9 and characterised TET protein expression in distinct

Thorold Theunissen

First naive human cells may offer an enhanced starting point for differentiation into disease-relevant cell types overcoming the heterogeneity frequently observed in current human ESCs and iPSCs Second the isolation of naive human cells may provide a cell culture system to study epigenetic mechanisms of human preimplantation development that cannot be investigated in primed cells

Another control element to the Nrf2 pathway is the transcriptional repressor Bach1 which can bind ARE enhancers blocking Nrf2 until naive cells are stimulated by pro-oxidants 15 16 Consequently under normal cellular conditions Nrf2 is anticancerous because of induction of cytoprotective and detoxification genes that protect cells from electrophilic/oxidative damage

Transcriptional Heterogeneity in Naive and Primed Human Pluripotent Stem Cells at Single-Cell Resolution Author links open overlay panel Tobias Messmer 1 2 7 Ferdinand von Meyenn 3 4 7 8 Aurora Savino 3 Ftima Santos 3 Hisham Mohammed 3 9

Primed Pluripotent Cell Lines Derived from Various Embryonic Origins and Somatic Cells in Pig Reinforcement of STAT3 activity reprogrammes human embryonic stem cells to naive-like pluripotency Epigenetic differences between nave and primed pluripotent stem cells

22-1-2019Transcriptional Heterogeneity in Naive and Primed Human Pluripotent Stem Cells at Single-Cell Resolution Tobias Messmer 1 2 7 Ferdinand von Meyenn 3 4 7 8 Aurora Savino 3 Ftima Santos 3 Hisham Mohammed 3 9 Tin Long Lun 1 ∗

Food can trigger a diverse array of symptoms in food allergic individuals from isolated local symptoms affecting skin or gut to multi-system severe reactions (systemic anaphylaxis) Although we know that gastrointestinal and systemic manifestations of food allergy are mediated by tissue mast cells (MCs) it is not clear why allergen exposure by the oral route can result in such distinct

Our study provides important insights into the transcriptomic heterogeneity of naive and primed hESCs The identification of specific markers may contribute to studying the reprogramming dynamics during the primed-to-naive transitions and delineate key transcriptional events leading to human naive pluripotency

Transcriptional profiling identifies caspase-1 as a T cell–intrinsic regulator of Th17 differentiation The frequency of naive precursors for specific epitopes is extremely low Functional heterogeneity of human memory CD4 + T cell clones primed by pathogens or vaccines

Lineage tracing on transcriptional landscapes links state

Biologists have long attempted to understand how stem and progenitor cells in regenerating and embryonic tissues differentiate into mature cell types Through the use of recent technical advances to sequence the genes expressed in thousands of individual cells differentiation mechanisms are being revealed Weinreb et al extended these methods to track clones of cells (cell families) across time

Primed Pluripotent Cell Lines Derived from Various Embryonic Origins and Somatic Cells in Pig Reinforcement of STAT3 activity reprogrammes human embryonic stem cells to naive-like pluripotency Epigenetic differences between nave and primed pluripotent stem cells

BackgroundHuman pluripotent stem cells (hPSCs) provide powerful models for studying cellular differentiations and unlimited sources of cells for regenerative medicine However a comprehensive single-cell level differentiation roadmap for hPSCs has not been achieved ResultsWe use high throughput single-cell RNA-sequencing (scRNA-seq) based on optimized microfluidic circuits to profile early

Pluripotency defines the propensity of a cell to differentiate into and generate all somatic as well as germ cells The epiblast of the early mammalian embryo is the founder population of all germ layer derivatives and thus represents the bona fide in vivo pluripotent cell population The so-called pluripotent state spans several days of development and is lost during gastrulation as

2 Transition from naive pluripotency to lineage specification in the embryo Between embryonic day 4 and 5 the mouse ICM segregates into two compartments: the naive epiblast and the hypoblast or primitive endoderm (PrE) The epiblast subsequently develops into the embryo proper whereas PrE gives rise to extraembryonic yolk sac tissues

21-6-2020(2017) Sahakyan et al Cell Stem Cell Naive human embryonic stem cells (hESCs) can be derived from primed hESCs or directly from blastocysts but their X chromosome state has remained unresolved Here we show that the inactive X chromosome (Xi) of primed hESCs was reactivated in naive culture c

The ability of human pluripotent stem cells (PSCs) to give rise to all of the cells in our body from neurons to kidney cells has fascinated researchers in developmental biology and regenerative medicine PSCs exist in two dynamic states nave or primed and recapitulate an early or late stage of embryonic development respectively